dr Katarzyna Jastrzębska

Adiunkt, Dział Chemii Bioorganicznej

Telefon: (+48) (42) 68 03 215

Email:

Strona www

Education:

• 2017: Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Department of Bioorganic Chemistry.
  Ph.D. Thesis: Conformation and thermodynamic stability of complexes of stereodefined PS-DNA/LNA oligomers with complementary DNA and RNA templates. Supervisor: Dr. Piotr J. Guga
• 2010: University of Lodz, Department of Chemistry.
  M.Sc. Thesis: Synthesis of liquid crystals based on verdazyl and benzo[1,2,4]triazinyl radicals. Supervisors: Prof. Piotr Kaszynski (Vanderbilt University), Prof. Zbigniew H. Kudzin (University of Lodz)

Research experience:

• 2010 – present: CMMS PAS, Department of Bioorganic Chemistry. Participation in projects funded by the NSC:
• SONATA 17 (2021/43/D/ST4/02433): P-stereodefined phosphorothioate analogs of “morpholino” oligonucleotides (sTMO) as a new model of therapeutic nucleic acids: synthesis, structure, physicochemical and biological properties. Principal Investigator.
• OPUS 10 (2015/19/B/ST5/03116): Double stranded probes, in which both oligonucleotide components hybridize according to the Hoogsteen’s interactions, and containing P-stereodefined phosphorothioate oligonucleotides made of DNA, RNA, (2′-OMe)-RNA and/or LNA units – synthesis and hybridizing properties towards DNA and RNA matrices. Co-Investigator.
• OPUS 6 (2013/11/B/ST5/01581): P-Stereodefined phosphorothioate analogs of DNA containing within nucleobases modifications preventing Watson-Crick base pairings – Hoogsteen interactions with RNA and DNA templates. Co-Investigator.
• OPUS 2 (2011/03/B/ST5/02670): Stereodefined phosphorothioate Glycol Nucleic Acids (GNA) – synthesis and physicochemical properties. Co-Investigator.
• OPUS 2 (2011/03/B/ST5/02671): Stereodefined phosphorothioate analogues of DNA containing LNA units – synthesis and thermodynamic properties of complexes formed by stereodefined PS-Oligos with complementary DNA and RNA templates. Co-Investigator.
• 2018-2019 (18 months): Postdoctoral fellow, University of Colorado at Boulder (CO, USA), Department of Chemistry and Biochemistry. Professor Marvin H. Caruthers’ Laboratory.
• 2009 (6 months): M.Sc. Student, performing an experimental part of master’s thesis, Vanderbilt University, Department of Chemistry. Professor Piotr Kaszynski Laboratory.

Publications:

Le B. T., Paul S., Jastrzebska K., Langer H., Caruthers M.H., Veedu R.N., Thiomorpholino oligonucleotides as a robust class of next generation platforms for alternate mRNA splicing, PNAS, 2022, 119, 1-8
https://www.pnas.org/doi/10.1073/pnas.2207956119

Jastrzębska K., Maciaszek A., Dolot R., Tomaszewska-Antczak A., Mikołajczyk B., Guga P., Synthesis and hybridizing properties of P-stereodefined chimeric [PS]-{DNA:RNA} and [PS]-{DNA:(2′-OMe)-RNA} oligomers, RSC Adv., 2022, 12, 26815-26824
https://pubs.rsc.org/en/content/articlelanding/2022/ra/d2ra04855h

Dumbović G., Braunschweig U., K. Langner H., Smallegan M., Biayna J., Hass E.P., Jastrzebska K., Blencowe B., Cech T.R., Caruthers M.H., Rinn J.L., Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention, Nature Comm. 2021, 12, 3308, 1-19
https://www.nature.com/articles/s41467-021-23221-w

Langner H., Jastrzebska K., Caruthers M., Synthesis and Characterization of Thiophosphoramidate Morpholino Oligonucleotides and Chimeras, J. Am. Chem. Soc. 2020, 142, 38, 16240–16253
https://pubs.acs.org/doi/10.1021/jacs.0c04335

Jastrzębska K., Mikołajczyk B., Guga P., LNA units present in [RP-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2′-OMe)-RNA strands, RSC Adv., 2020, 10, 22370-22376
https://pubs.rsc.org/en/content/articlelanding/2020/ra/d0ra03934a

Krishna H., Jastrzebska K., Caruthers M., Exploring site-specific activation of bis-N,N’-dialkylaminophosphordiamidites and the synthesis of morpholinophosphoramidate oligonucleotides, FEBS Lett., 2019, 593, 1459-1467
https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13492

Maciaszek A., Jastrzębska K., Guga P., Homopurine RP-stereodefined phosphorothioate analogs of DNA with hampered Watson-Crick base pairings form Hoogsteen paired parallel duplexes with (2′-OMe)-RNAs, Org. Biomol. Chem., 2019, 17, 4611-4620
https://pubs.rsc.org/en/content/articlelanding/2019/ob/c8ob03112f

Tomaszewska-Antczak A., Jastrzębska K., Maciaszek A., Mikołajczyk B., Guga P., P-Stereodefined phosphorothioate analogs of glycol nucleic acids – synthesis and structural properties, RSC Adv., 2018, 8, 24942-24952
https://pubs.rsc.org/en/content/articlelanding/2018/ra/c8ra05568h

Jastrzębska K., Maciaszek A., Dolot R., Bujacz G., Guga P., Thermal Stability and Conformation of Antiparallel Duplexes Formed by P-Stereodefined Phosphorothioate DNA/LNA Chimeric Oligomers with DNA and RNA Matrices, Org. Biomol. Chem., 2015, 13, 10032–10040
https://pubs.rsc.org/en/content/articlelanding/2015/ob/c5ob01474c

Jasiński M., Gerding J.S., Jankowiak A., Gębicki K., Romański J., Jastrzębska K., Sivaramamoorthy A., Mason K., Evans D.H., Celeda M., Kaszyński P., Functional Group Transformations in Derivatives of 6-Oxoverdazyl, J. Org. Chem., 2013, 78, 7445-7454
https://pubs.acs.org/doi/10.1021/jo400843y

Patents:

WO/2021/155120 (USA): Dumbović G. (CRO/US), Krishna H. (IN/US), Jastrzebska K. (PL/US), Caruthers M. (US), Rinn J.L. (US), Method for retaining splicing RNAs in the nucleus based on chemically modified antisense Oligonucleotides

WO/2019/060522 (USA): Krishna H. (IN/US), Jastrzebska K. (PL/US), Caruthers M. (US), Paul S. (US), Veedu R.N. (AU), Thiomorpholino oligonucleotides for the treatment of muscular dystrophy