dr inż. Wojciech Cypryk

Adiunkt, Dział Chemii Bioorganicznej

Telefon: (+48) (42) 68 03 215

Email:

Pokój: Budynek C, pokój 104

Scientific career:

• 2010 – MSc (Biotechnology) – Technical University of Denmark, Technical University of Lodz
• 2016 – PhD (Biotechnology) – University of Helsinki (Finland)
• 2016 – onwards – specialist and group leader at CMMS PAS

Current research area:

I have been studying human innate immunity, with particular interest in biogenesis of extracellular vesicles (EV) and their roles in mediating inflammatory responses.  The key questions of my research are how intracellular signaling pathways cross-talk in generation of EV and how are  particular cargos recruited to secreted vesicles. This is particularly relevant for understanding the roles vesicle-mediated signal transduction in immune-related diseases, such as inflammation, cancer etc. Currently I am involved in research covering the secretion and composition modulation of EV secreted in various models of inflammation in human primary cells (National Science Centre grant no. 2018/28/C/NZ6/00069).

• Canonical and non-canonical inflammasome signaling in human leukocytes
• Proteomics of extracellular vesicles
• Novel signaling in regulation of inflammatory responses and EV secretion

Membership in scientific organizations:

• 2012 – 2016 – Finnish Proteomics Society (FinProt)
• 2016 – onwards – Polish Biochemical Society (PTBioch)

Publications:

• Matikainen S, Nyman TA, Cypryk W (2020) Function and regulation of noncanonical caspase-4/5/11 inflammasome, J Immunol 204 (12), 3063-3069.
• Kluszczyńska K, Czernek L, Cypryk W, Pęczek Ł, Düchler M (2019) Methods for the determination of the purity of exosomes, Curr Pharm Des 25 (42), 4464-4485.
• Düchler M, Czernek L, Pęczek L, Cypryk W, Sztiller-Sikorska M, Czyż M (2019) Melanoma-derived extracellular vesicles bear the potential for the induction of antigen-specific tolerance, Cells 8 (7), 665.
• Cypryk W, Nyman TA, Matikainen S (2018) From inflammasome to exosome: does extracellular vesicle secretion constitute an inflammasome-dependent immune response? Front Immunol 9, 2188.
• Nyman TA, Lorey MB, Cypryk W, Matikainen S (2017) Mass spectrometry-based proteomic exploration of the human immune system: focus on the inflammasome, global protein secretion, and T cells. Expert Rev Proteomics 14, 395-407.
• Møller MS, Goh YJ, Rasmussen KB, Cypryk W, Celebioglu HU, Klaenhammer TR, Svensson B, Abou Hachem M (2017) An extracellular cell-attached pullulanase confers branched α-glucan utilization in human gut Lactobacillus acidophilus, Appl Environ Microbiol 83, pii: AEM.00402-17.
• Cypryk W, Lorey M, Puustinen A, Nyman TA, Matikainen S (2017) Proteomic and bioinformatic characterization of extracellular vesicles released from human macrophages upon influenza A virus infection. J Proteome Res 16, 217-227.
• Välimäki E, Cypryk W, Virkanen J, Nurmi K, Turunen PM, Eklund KK, Åkerman KE, Nyman TA, Matikainen S (2016) Calpain activity is essential for ATP-driven unconventional vesicle-mediated protein secretion and inflammasome activation in human macrophages. J Immunol 197, 3315-3325.
• Cypryk W, Öhman T, Eskelinen EL, Matikainen S, Nyman TA, (2014) Quantitative proteomics of extracellular vesicles released from human monocyte-derived macrophages upon β-glucan stimulation. J Proteome Res 13, 2468-2477.
• Öhman T, Teirilä L, Lahesmaa-Korpinen AM, Cypryk W, Veckman V, Saijo S, Wolff H, Hautaniemi S, Nyman TA, Matikainen S (2014) Dectin-1 pathway activates robust autophagy-dependent unconventional protein secretion in human macrophages. J Immunol 192, 5952-5962.